Coding

Part:BBa_K4065005:Design

Designed by: Raúl Acosta Murillo   Group: iGEM21_FCB-UANL   (2021-08-14)


cas9 type II [Streptococcus pyogenes]


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1340
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1340
    Illegal NheI site found at 1099
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1340
    Illegal BamHI site found at 3378
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1340
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1340
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Original sequence.


Source

cas9 type II CRISPR RNA-guided endonuclease Cas9 [Streptococcus pyogenes] - Gene - NCBI. (2021). Nih.gov. https://www.ncbi.nlm.nih.gov/gene/57852564

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References